Effect of Xiaoaiping on the expression of circadian clock genes in human hepatoma HepG2 cells

Effect of Xiaoaiping on the expression of circadian clock genes in human hepatoma HepG2 cells

Meng-Meng Wang1, #, Jing Xu2, #, Xiao-Xiao He1, Meng-Jun Qiu1, Zhi-Fan Xiong1,*, Sheng-Li Yang3,*

【摘 要】Abstract Objective: Investigation of the effect of Xiaoaiping on the expression of circadian clock genes in human hepatoma HepG2 cells. Methods: Selecting the HepG2 cells in the logarithmic growth phase and assigning them to Xiaoaiping injection (XAP)group and control group. The two groups were treated with 75 mg/mL XAP or the same dose of normal saline. After 72 h of treatment, real-time PCR was used to detect the expression of circadian clock genes in HepG2 cells and Western Blot technology was used to detect the expression of related proteins. Results: The mRNA expression levels of PER1, NPAS2, NR1D1, and DEC1 in the XAP group was significantly higher than that in the control group (P< 0.05), while the mRNA expression levels of PER3, BMAL1, DEC2, and RORA were significantly lower in the XAP group than in the control group (P < 0.05), and there was no significant difference between the mRNA expression levels of PER2, CRY1, CRY2, and TIM.Of course, the proteins’ expression levels of the genes we had detected such as PER1~3, CRY1~2, CLOCK, BMAL1 by Western Blot were consistent with the real-time PCR results above. Conclusion: XAP affects the expression of circadian clock genes in HepG2 cells.