Donor MHC gene to mitigate rejection of
transplantation in recipient mice
LI Tong;ZHANG Zhi-tai;LI Hui;YAN Jun;TAN Jia-li;L(U) Yue-ping;HOU Sheng-cai;LI Shen-tao;XU Qing;TONG Xue-hong;DING Jie
【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2011(124)024
【摘要】Background Donor organ rejection continues to be a significant problem for patients receiving transplants.We therefore tested whether transferring a donor's major histocompatibility complex (MHC) gene to the recipient would mitigate the rejection of transplanted hearts in mice.Methods H-2Kkgene from donor mice was amplified using nested polymerase chain reaction (PCR) and ligated into a mammalian expression vector,which was then transfected into thymus ground mass cells collected from the recipients.Clones stably expressing the transgene were then injected into the recipients' thymus visualized using ultrasound.Control mice were administered cells previously transfected with empty vector.Following heart transplantation,cardiac activity was monitored electrocardiographically.Recipient thymus cells were tested for MHC antigenicity using flow cytometry and spleen cells were subjected to mixed lymphocyte culture tests.Finally,the transplanted hearts were sectioned,stained and examined under light microscopy.Results Southern analysis following nested PCR revealed
Donor MHC gene to mitigate rejection of transplantation in recipient mice
