CO2气腹对腹膜超微结构的影响
王胜;陈海亮;胡芳
【期刊名称】《中国现代普通外科进展》 【年(卷),期】2011(014)010
【摘要】目的:探讨二氧化碳(CO2)气腹腹腔镜手术对人体腹膜形态学的影响.方法:对80例胆囊炎患者随机分别进行腹腔镜手术和传统开腹手术,于手术前及气腹后30 min分别采集壁层腹膜,对其进行扫描电镜(SEM)检测观察腹膜间皮细胞形态学变化.结果:传统手术组开腹后间皮细胞未见明显变化,细胞形态正常,基底膜完整,关腹前腹膜结构仍然未出现明显形态学改变.腹腔镜手术组CO2低压充气30 min后可见出现腹膜间皮细胞肿胀,高压充气30 min可见腹膜间皮细胞间连接断裂基底膜裸露,细胞间隙可见,少量淋巴细胞和巨噬细胞.结论:CO2气腹腹腔镜手术可使腹膜形态学发生明显变化,可能是腹腔镜手术时造成肿瘤细胞发生腹膜转移的原因.%Objective: To investigate characteristic alterations of the peritoneum after CO2 pneumoperitoneum used in laparoscopic surgery. Methods: Eighty patients with acute cholecystitis were randomly divided into 2 equal groups to undergo laparoscopic surgery with CO2 pneumoperitoneum or laparotomy respectively. Specimens of parietal peritoneum were obtained at ap-propriate time points to undergo scanning electronmicroscopy to observe the morphological changes. Results: In the laparotomy group, there was no marked change. However, in the CO2 pneumoperitoneum group bulging up of mesothelial cells was evident immediately at the time of filling of CO2,
CO2气腹对腹膜超微结构的影响
