The ruminal bacterial community in lactating dairy cows has limited variation on a day-to-day basis

The ruminal bacterial community in lactating dairy cows has limited variation on a day-to-day basis

Joseph H.Skarlupka1,Maria E.Kamenetsky2,3,Kelsea A.Jewell4 and Garret Suen1*【摘 要】Abstract Dairy cows rely on a complex ruminal microbiota to digest their host-indigestible feed. Our ability to characterize this microbiota has advanced significantly due to developments in next-generation sequencing. However, efforts to sample the rumen, which typically involves removing digesta directly from the rumen via a cannula, intubation, or rumenocentesis,is costly and labor intensive.As a result,the majority of studies characterizing the rumen microbiota are conducted on samples collected at a single time point. Currently, it is unknown whether there is significant day-to-day variation in the rumen microbiota,a factor that could strongly influence conclusion drawn from studies that sample at a single time point. To address this, we examined day-to-day changes in the ruminal microbiota of lactating dairy cows using next-generation sequencing to determine if single-day sampling is representative of sampling across 3 consecutive days. We sequenced single-day solid and liquid fractions of ruminal digesta collected over 3 consecutive days from 12 cannulated dairy cows during the early, middle, and late stages of a single lactation cycle using the V4 region of the bacterial 16S rRNA gene. We then generated 97% similarity operational taxonomic units (OTUs) from these sequences and showed that any of the individual samples from a given 3-day sampling period is equivalent to the mean OTUs determined from the combined 3-d data set.This finding was consistent for both solid and liquid fractions of the rumen,and we thus conclude that there is limited day-to-day variability in the rumen microbiota.【期刊名称】《畜牧与生物技术杂志(英文版)》【年(卷),期】2019(010)004【总页数】5

【关键词】Keywords:Bacterial community,Next-generation sequencing,Rumen microbiota*Correspondence:gsuen@wisc.edu

1Department of Bacteriology, University of Wisconsin-Madison, 5159 MSB,1550 Linden Drive, Madison, WI 53706,USA

Full list of author information is available at the end of the article