海金沙不同溶剂提取物清除自由基活性的研究
贲永光;李康;李坤平;张伟永
【期刊名称】《安徽农业科学》 【年(卷),期】2009(035)019
【摘要】[目的]比较不同溶剂提取海金沙总黄酮对自由基活性清除能力的大小.[方法]应用DPPH 法、邻苯三酚自氧化法和Fenton反应分别研究了浓度95%乙醇、乙酸乙酯、丙酮、乙酸、氯仿、甲醇提取物清除自由基活性的能力.[结果]6种溶剂提取得到的海金沙提取物对DPPH·、OH·和O2-·均有一定程度的清除作用, 不同溶剂所得提取物对自由基的清除作用均有差别,其中浓度95%乙醇提取得到的海金沙提取物对3种自由基清除效果均最好.[结论]海金沙可作为抗氧化物质资源进行开发利用.%[Objective] The research aimed to compare the scavenging abilities of total flavone from Lygodium japonicum with different solvents to free radical activity.[Method] DPPH method, pyrogallol method and Fenton reaction were used to study the scavenging ability of extracts with 95% ethanol, ethyl acetate, acetone, acetic acid, chloroform, methanol to free radical activity.[Result] Six kinds of solvent extracts from L.japonicum had certain scavenging ability to DPPH·, OH · and O2-·.The scavenging ability of extracted with different solvents to free radicals were difference.The extracts from L.japonicum with 95% ethanol had the best scavenging effects on three kinds of free radicals.[Conclusion] L.japonicum can be used as antioxidants for the development and utilization of resources.
海金沙不同溶剂提取物清除自由基活性的研究
