体内阿霉素干预富集肝癌干细胞的初步研究
佚名
【期刊名称】《中国癌症防治杂志》 【年(卷),期】2013(000)002
【摘要】 目的建立肝癌裸鼠皮下移植瘤动物模型，以癌干细胞的耐药特性为建立富集肝癌干细胞的切入点，在机体环境下进行构建体内肝癌干细胞的富集模式。方法将BEL-7404人肝癌细胞株的细胞悬液注射裸鼠两侧腋窝皮下，建立皮下移植瘤模型，经阿霉素干预后原代取材，分别以单个细胞克隆形成实验、平板克隆形成实验和悬浮球形成实验，对亲本细胞及经阿霉素干预后的BEL-7404肝癌细胞进行癌干细胞相关细胞学特性的检测。结果每一代裸鼠皮下移植瘤模型成瘤率为100%，亲本细胞与经体内阿霉素干预后的第一代BEL-7404肝癌细胞的单细胞克隆形成率比较没有明显差异（P跃0.05），而体内干预第二代细胞的单细胞克隆形成率均明显高于亲本细胞和经干预后的第一代细胞（P约0.05），体内干预第一代细胞平板克隆形成率均明显低于亲本细胞和第二代细胞（P约0.05）。亲本细胞及经体内阿霉素干预后的BEL-7404肝癌细胞在干细胞培养基中培养均未见有细胞悬浮球形成。结论经低剂量阿霉素体内干预可作为富集肝癌干细胞的手段之一。%Objective To establish a nude mouse model of liver cancer and explore whether drug resistance of cancer stem-like cells in vivo can lead to enrichment of stem-like cells. Methods A subcutaneous tumor model of liver carcinoma was established by subcu-taneously injecting human liver BEL-7404 cell suspensions into both axilla of BALB/C-nu mice.Following adriamycin
treatment,pri-mary cultures were established,and both parental and adriamycin-treated cells were studied in assays of single-cell colony formation and sphere cell formation to detect drug resistance and analyze other cytological characteristics. Results The tumor formation rate in this BALB/C-nu subcutaneous tumor model was 100%.There were no significant differences in the rate of single-cell colony formation between BEL-7404 parental liver cells and first-generation cells after adriamycin treatment(P>0.05).However,second-generation cells showed a significantly higher rate of single-cell colony formation and a significantly lower colony formation rate than parental or first-generation cells(P<0.05). Non-adherent spheres were not observed in cultures of any of the three cell types. Conclusions Low-dose adriamycin intervention may be a way to enrich for liver cancer stem-like cells in vivo.
【总页数】4页(92-94,95)
【关键词】肝肿瘤;癌干细胞;皮下移植瘤模型;阿霉素 【作者】佚名 【作者单位】 【正文语种】中文 【中图分类】R735.7 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-
journal-oncology-prevention-treatment_thesis/0201236611647.html 【相关文献】
1.表阿霉素和阿霉素诱导人肝癌细胞凋亡 [J], 马祖胜; 冯英明; 姬统理; 张贺龙; 唐敏章; 王文亮; 郭双平
2.阿霉素诱导人肝癌细胞株HCC—9204肝癌细胞凋亡的形态学 … [J], 张晓东; 王文亮
3.鼠抗人肝癌单抗Hab18—葡聚糖—阿霉素结合物在荷瘤裸鼠体内的… [J], 何志龙; 刘彦仿
4.阿霉素免疫毫微粒对多药耐药肝癌的体内杀伤作用 [J], 阚和平; 王宇; 谭永法; 周杰
5.阿霉素不同剂型经肝动脉给药治疗肝癌的体内药动力学研究 [J], 韩国宏; 郭宇良
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体内阿霉素干预富集肝癌干细胞的初步研究
