双链DNA裂解-纳米金共振散射光谱探针检测痕量
UO2^2＋
张轶;梁爱惠;周莲平;覃惠敏;欧阳辉祥;王鹏飞;蒋治良
【期刊名称】《化学学报》 【年(卷),期】2011(069)018
【摘要】In pH 5.5 2-（N-morpholine）-ethyl sulfonic acid buffer solution and in the presence of NaCl,the substrate single stranded DNA hybridized with the DNA enzyme to form double stranded DNA（dsDNA） at 80 ℃.UO22＋ can cleaved the substrate strand of the dsDNA to produce short single stranded DNA that adsorbed on the surface of the gold nanoparticle to prevent its aggregation,and the uncombined gold nanoparticles aggregate to big particles that exhibited a resonance scattering（RS） peak at 610 nm.When the UO22＋ concentration increased,the short single stranded DNA increased,the combined gold nanoparticles increased,and the aggregated gold nanoparticles decreased,the RS intensity at 610 nm decreased.Under the selected conditions,the decreased RS intensity（ΔI610 nm） is linear to UO22＋ concentration in the range of 0.67～60.3 nmol/L,with a regression equation of ΔI610 nm=11.9C＋6.1,a correlation coefficient of 0.9972,and a detection limit of 0.09 nmol/L UO22＋.This method has been applied to determination of UO22＋ in wastewater,with satisfactory results.%在pH 5.5的2-（N-吗啉）-乙磺酸缓冲液中,底物DNA
双链DNA裂解-纳米金共振散射光谱探针检测痕量UO2^2+
