STAT1基因敲除提高小鼠对EV71病毒的敏感性研究
李光超;张倩;武婧;赵彬彬;陈鑫;秦川;刘江宁
【期刊名称】《中国医药导报》 【年(卷),期】2017(014)015
【摘要】目的 分析STAT1基因敲除小鼠免疫相关因子表达差异,观察STAT1基因敲除小鼠感染EV71病毒后的症状表现及病毒载量,为EV71抗病毒治疗提供数据基础.方法 LPS刺激STAT1基因敲除小鼠后,利用微珠免疫分析(CBA)方法对血清免疫相关因子定量分析;EV71感染STAT1基因敲除小鼠后,观察症状及病理表现,利用实时荧光定量检测对肌肉病毒RNA定量分析.结果 LPS刺激后,STAT1基因敲除小鼠血清多种细胞因子分泌水平下降(P＜ 0.01);EV71感染后,STAT1基因敲除小鼠病理损伤加重,肌肉中病毒载量升高(P＜0.01),与野生型小鼠相比表现出更严重的症状与更高的死亡率.结论 STAT1在抗EV71感染的免疫过程中扮演重要角色,提示STAT1基因敲除可以提高小鼠对EV71病毒的敏感性.%Objective To analyze the differential expression of immune-associated factors in STAT1 knockout mice,and observe the symptoms and viral load after EV71 virus infection,in order to provide a data base for anti-EV71 therapy.Methods Cytometric bead assay (CBA) was used to quantitative analysis the levels of serum immune-associated factors in STAT1 knockout mice after LPS-induced treatment.The symptom and tissue pathological features were observed and the level of muscle virus RNA was quantitated by real-time PCR in STAT1 knockout mice after EV71 virus infection.Results The levels of multi-immune-associated
STAT1基因敲除提高小鼠对EV71病毒的敏感性研究
