生长抑素对肝硬化大鼠肝切除术后小体积肝的保护作用
徐勇;任祖海;俞照妹;李岳
【期刊名称】《中华肝胆外科杂志》 【年(卷),期】2017(023)008
【摘要】目的 探讨生长抑素对肝硬化大鼠肝大部切除术后小体积肝的保护性治疗作用.方法 48只肝硬化大鼠随机分成生长抑素治疗组(S组,n =24)和生理盐水对照组(N组,n=24).两组大鼠常规测量基础门静脉压力及血流量,分别于肝门阻断时以及缺血再灌注后15、30、60 min测量门静脉压力及血流量,于再灌注1、3、5、24h抽血行肝功能检测.结果 再灌注后15 min,S组大鼠门静脉压力低于N组[(19.4±0.8)cmH2O比(22.5±1.2)cm H2O(1 cmH2O =0.098 kPa)],差异有统计学意义(P＜0.05).再灌注后30 min,S组与N组门静脉压力分别为(17.1±0.8) cmH2O及(19.7±0.8) cmH2O,差异有统计学意义(P＜0.05).两组大鼠各个时点门静脉血流量均高于基础血流量.再灌注后15 min,门静脉血流量升高最为明显,S组血流量低于N组[(10.1±0.4)ml/min比(11.9±0.5) ml/min],差异有统计学意义(P＜0.05).两组大鼠AST、ALT、TBil均高于正常,且随时间延长呈上升趋势.术后1周大鼠存活情况,S组(7/10)优于N组(2/10),差异有统计学意义(P＜0.05).两组大鼠的残余肝脏重量均有所增加,S组较N组残肝重量增加明显[(5.5±0.4)g比(6.6±0.3)g,P＜0.05].结论 生长抑素能有效缓解肝硬化大鼠肝切除术后小体积肝的早期门静脉高压状态,降低术后门静脉血流量.减轻门静脉高压及门脉高灌注对小体积肝的损伤可促进肝再生,改善肝硬化大鼠术后的存活情况.%Objective To explore the role of somatostatin in protecting small-for-size liver after hepatectomy in rats with cirrhosis.Methods Forty-
eight rats with cirrhosis were randomly divided into somatostatin group (group S,n =24) and normal saline control group (group N,n =24).All rats from the two groups were measured with baseline portal vein pressure and blood flow volume.Additionally,at the endpoints of 15,30 and 60 min after ischemia/reperfusion with hepatic inflow occlusion,portal vein pressure and blood flow volume were detected from both groups.Liver function test was also measured at the endpoints of 1,3,5,24 h after ischemia/reperfusion for groups S and N.Results After 15-min ischemia/reperfusion,the portal vein pressure was higher in the rats of group S than that in group N [(19.4 ± 0.8) cmH2O vs.(22.5 ± 1.2) cmH2O (1 cmH2O =0.098 kPa)],there was significant
difference
(P
＜
0.05).After
30
min
ischemia/reperfusion,portal vein pressure results were (17.1 ± 0.8) cmH2O and (19.7 ± 0.8) cmH2O in group S and group N,respectively,P ＜ 0.05.At different endpoints,portal blood flow volume results were all higher in both groups than baseline figures.Furthermore,maximum portal blood flow volume was observed at the endpoint of 15 min ischemia/reperfusion in both groups,and lower in group S than group N [(10.1 ±0.4)ml/min vs.(11.9 ±0.5)ml/min,P＜0.05].Liver function test revealed AST,ALT,TBil exceed normal limits in rats of both groups,which elevated along prolonged ischemia/reperfusion.One week after hepatectomy,the survival of rats treated with somatostatin (group
S,7/10) was superior to saline control group (group N,2/10),P ＜0.05.Future liver volume increased in rats from both groups,much significant increase was determined in group S [(5.5 ± 0.4) g vs.(6.6 ± 0.3) g,P ＜ 0.05].Conclusions Somatostatin might improve portal vein hypertension status in the early stage of small-for-size liver after hepatectomy,and decrease portal blood flow.Furthermore,it could alleviate small-for-size liver injury associated with portal vein hypertension and hyperperfusion,and benefit liver regeneration and improve postoperative survival in rats with cirrhosis. 【总页数】6页(542-547)
【关键词】生长抑素;肝硬化;肝切除术;小体积肝 【作者】徐勇;任祖海;俞照妹;李岳
【作者单位】410000长沙,中南大学湘雅三医院血液净化中心;410000长沙,中南大学湘雅三医院普外科;410000长沙,中南大学湘雅三医院普外科;410000长沙,中南大学湘雅三医院普外科 【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-hepatobiliary-surgery_thesis/0201234571969.html 【相关文献】
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生长抑素对肝硬化大鼠肝切除术后小体积肝的保护作用
