氩氦冷冻治疗后的荷C6胶质瘤大鼠的磁共振成像和病理研
究
李明;黄凡衡;胡丽娟;周媛;姜晓丹;常海刚;卢凤飞;陈强;张世忠
【期刊名称】《中华神经医学杂志》 【年(卷),期】2010(009)004
【摘要】目的 探讨荷C6胶质瘤大鼠经氩氦刀治疗后的影像学和病理学的变化. 方法 将51只Wistar大鼠背部皮下植入体外扩增的C6胶质瘤细胞,制作荷C6胶质瘤的动物模型,模型制作成功后按照随机数字表法分为假手术组(暴露肿瘤后只插入冷冻刀头不启动氩氦冷冻系统,n=18),外科手术切除组(将肿瘤完整切除,不进行冷冻操作,n=15)和氩氦冷冻组(暴露肿瘤后启动氩氦冷冻系统,n=18).观察各组大鼠治疗前后MRI影像变化,应用Tunel检测细胞凋亡情况.结果 外科手术成功切除胶质瘤.氩氦冷冻组MRI显示T1、W1信号较术前增强,T2WI信号较术前减低假手术组治疗后MRI无明显变化.氩氦冷冻组肿瘤组织Tunel染色可见大量凋亡细胞,外科手术切除组及假手术组可见少量散在的凋亡细胞.结论 经氩氦刀治疗胶质瘤MRI提示肿瘤细胞变性坏死,病理学提示可以介导细胞凋亡,有望成为脑胶质瘤的一种有效的辅助治疗手段.%Objective To explore the changes of imaging and pathologies in rots with bearing C6 gliomas after cryotherapy. Methods Animal models with subcutaneously transplanting C6 gliomas in the Wistar rates were established. Fifty-one rat models were randomized into sham-operated group (n=18), resection group (n=lS) and cryotherapy group (n=18). The tumors in the sham-operated group were exposed only and the tumors in the
氩氦冷冻治疗后的荷C6胶质瘤大鼠的磁共振成像和病理研究
