Comparative transcriptome analysis of the effect of different heat shock periods on the un

Comparative transcriptome analysis of the effect of different heat shock periods on the unfertilized

ovule in watermelon(Citrullus lanatus)

ZHU Ying-chun; SUN De-xi; DENG Yun; AN Guo-lin; LI Wei-hua; SI Wen-jing; LIU Jun-pu; SUN Xiao-wu

【期刊名称】《农业科学学报：英文版》 【年(卷),期】2020(019)002

【摘要】In vitro gynogenesis is an important tool used in haploid or homozygous double-haploid plant breeding.However,because of low repeatability,embryoid induction rate and quality,the molecular mechanisms remain poorly understood.Heat shock treatment can promote the transformation of the gametophytic pathway into the sporophyte pathway,which induces the occurrence of haploid.In this study,unfertilized ovaries were heat shocked for 0 h(A0)before flowering and for 0 h(A1),4 h(A3),8 h(A5),12 h(A7),and 24 h(A8),respectively,at 37℃at the first day of the flowering stage.The ovule enlargement rate was increased from 0%at 25℃to 96.8%at 37℃(24 h treatment).Thus,we aimed to investigate the gene expression patterns in unfertilized ovules of watermelon after different periods of heat shock by using RNA-Seq technology.The results showed that compared with A3,A5,A7,and A8,the biosynthesis of amino acid,glycine,serine and threonine metabolic pathways in A1 has changed significantly.This indicated that heat shock

treatment affected the synthesis and transformation of amino acids during ovule expansion.The transcriptome data suggested gene expressions of ovule growth were significantly changed by heat-specific influences.The results provide new information on the complex relationship between in vitro gynogenesis and temperature.This provides a basis for further study of the mechanism of heat shock affecting the expansion of watermelon ovule. 【总页数】13页(P.528-540)

【关键词】watermelon; heat shock; unfertilized ovule; ovule enlargement; transcription factors

【作者】ZHU Ying-chun; SUN De-xi; DENG Yun; AN Guo-lin; LI Wei-hua; SI Wen-jing; LIU Jun-pu; SUN Xiao-wu

【作者单位】College of Horticulture Hunan Agricultural University Changsha 410128 P.R.China; Zhengzhou Fruit Research Institute Chinese Academy of Agricultural Sciences Zhengzhou 450009 P.R.China 【正文语种】中文 【中图分类】O62 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_journal-agricultural-sciences-english_thesis/0201278127823.html 【相关文献】

1.Comparative transcriptome analysis of the effect of different heat

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