生物学个人陈述
生物 2009-09-07 08:36:18 阅读224 评论0 字号：大中小
Why should I select biology as my career? Born in a medical family for generations, I was very interested in all over around, especially in living organisms and I came into a habit of watching, touching, feeling and protecting them who become my favorite friends later. Life science is much more intriguing and fascinating to me when I made endeavor to represent Shandong Province 400,000 high-school students to go to Beijing to take part in the National Biological Olympic Context, a Pick-Out for the International Biological Olympic Context. As a result, I selected Biotechnology, the foremost interesting field in Biology, as my college major when I won acceptance into Department of Biotechnology in the College of Life Science at Peking University, the best university of China.
Upon acceptance to Peking University in 1996, I was awarded consecutive scholarships for all 4 years in college. During my college years, with the systematic education, I obtained a solid academic background in chemistry and basic biology science especially in experiment skills as a preparation for future advanced research, which is also, the basis for me to do my current research project at the lab.
Due to my outstanding experiment skills, in the third year of my undergraduate study, I was recommended to the State Key Lab of Microbial Resource, Institute of Microbiology, Chinese Academy of Science, which only 2% the students can get in, to do research on the structure and character of a new family of DNA-Binding Protein from the Hyperthermophilic Archaeon Sulfolobus Shibatae(extreme environment). And independently I have been dedicating in the interaction between DNA and DNA-Binding Protein by Gel Retardation Assay, Nick Closure Assay and EMSA. This project provided me with not only considerable experiment experience including bacterial culture, expression of recombinant, and western blotting etc, but also how to plan an experiment, by consulting papers, seeking optimal conditions and selecting
appropriate methods. Now my partners and I have made a great achievement of this project and we have a paper, “A Highly Conserved DNA-Binding Protein from the Hyperthermophilic Archaeon Sulfolobus Shibatae Affects DNA Supercoiling in a Temperature-Dependent Fashion”, which will be published in the NUCLEIC ACIDS RESEARCH(4.488) in the nearer future.
Furthermore, I have once, as a co-operator, helped other group investigate Archaeon ssh7 DNA binding protein and DNA interaction. With the experiment process, I am proficient in PCR and RT-PCR, southern and northern blotting, cell in-situ blotting, CsCl density gradient centrifuge, expression of recombinant protein, FPLC, protein purification and protein/enzyme activity assay, etc.
In addition to academic studying and lab training, I benefit much from my college life. I joined the Green Life Association, one of the best student organizations in Peking University, and had been the minister of propagation for one year. The job has deepened my love for lives and improved my responsibility both for group and for the well-being of our earth. Moreover, all the many-sided pleasant experiences and excellent records are a great treasure to me, giving me high confidence and independence.
Why to go abroad to pursue Ph.D.? I’m always thinking that the research of life sciences is not only interdisciplinary but also international. Besides the advanced technology, I hope to fuse the analytic way of the east and west. So I’d like to go abroad for advanced studies after my graduation in July of 2000. And besides, it is to make more research achievement in the life science and at the same time, fulfill my keen dream of standing on the foremost frontline in biology science. Why to choose your University as my graduate school? It is that she is one of the most highly regarded universities in the world and is an ideal place for ambitious youth. Why to select your program? With my experiment skills and broad academic background, I am more and more certain that I am well prepared for the further exploration into your respected program, which is very promising in the modern biology. Despite the highly competition of your admission, I still regard your research program as my
future struggling goal.
At the time being, I think I am honest, intelligently mature and psychologically ready for this goal and I believe that I can accept the challenge and will surely make success in the end. Would you accept me as one of the members of your program to fulfill my dream? I hope I can contribute my strength and intelligence to your program. You are sure to find that your investment on me is really awarding and worthwhile.
Sincerely yours, Yunfei Wen
Biology - Personal Statement
生物 2009-09-07 08:35:52 阅读238 评论0 字号：大中小
By applying your Ph. D. program, I am seeking to fulfill a dream in my childhood, to be a great scientist. This dream encouraged me to read a lot of books on many disciplines, since my primary school time. Although my parents had to work abroad much of the time from then on and had no time to look into my study, I had never been slack, for the dream gave me a pressure to do my best in study.
In the meanwhile, my talent in mathematics helped me excel my classmates and gain outstanding grades. Besides my excellent grades in school, I won remarkable scores in various contests in Beijing and a few national contests on mathematics, physics, chemistry, etc. Moreover, from 1993 to 1998, I won the Shupin Scholarship several times based on my excellent score.
When I was in high school, I came to realize the great potential of life sciences. As we all know, mankind can go up to outer space or down to the bottom of the ocean, and predict the movement or state of a huge planet or an infinitesimal particle. Yet we can’t explain a lot of phenomena about ourselves or various lives around us, because lives are the most complicated systems in the world. I am sure that to explain these phenomena is what a great scientist should do, and just what I wish to do.
Therefore, when I was about to take the National College Entrance Examination, I chose the college of Life Sciences in Peking University, the most famous university in China, which is dubbed as China's Harvard by President Clinton on his visit to my country. In the university, I studied at the Department of Biochemistry & Molecular Biology, which required its students to have a great ability to learn and research. With the study of a series of courses, I obtained a solid academic background in chemistry and basic biology. At the same time, I learned many skills of both general chemistry experiments and specialized biology research. From lab courses, I experienced all kinds of experiments for DNA, protein and microbe’s research and analysis.
However, I realized that I need more practical experience before I step on the way to be a scientist. To enhance my research ability, I have been working as a research assistant since July 2000 in the Laboratory of Molecular Virology & Bioengineering at the Institute of Microbiology, Chinese Academy of Sciences (CAS) for a project on the regulation of cotton fertility in plant heterosis, which is part of the National High Technology Research & Development Program (the “863 Program”), and have got great progress in the work of clone, absence and analysis of promoter’s function on the specific expressed gene in
cotton pollen. We constructed plasmids that have pollen specific promoters and poisonous protein genes, and then transformed the recombinant genes into tobacco by agrobacterium or particle bombardment to study the long time and short time characteristics of them and their mutants under different conditions.
At the same time, I expressed a nuclease inhibitor gene, and purified the protein to prepare its antibody. With the antibody, I succeed in screening transformed cottons by immunoassays. This method can greatly improve the application of cotton’s heterosis, which has potential significance in our country’s cotton production. With some of the work above, I completed my undergraduate thesis, “High level expression of a nuclease inhibitor, Bastar in E.Coli and its application in screening transformed cotton”, and got a good score.
In addition, my skills gave me chances to be appointed to participate in the jobs of other projects, such as the study on the promoter and its function of specific expressed Annexin in strawberry fruit, and the translation of a book on Prion Biology and Disease. These jobs, which are in progress, gave me more exercises in various areas.
These studies and research experience provided me the first step to the way of being a biology scientist. Because they not only furthered my learning and experimental skills, but also greatly increased my interest in the field of Regulation of Gene Expression and its application in Protein's Structure and Function. I would like to continue my study in molecular biology and biochemistry, especially in the area of gene regulation, study on the character of DNA and protein, as well as DNA-protein interaction. Because I think they are the foundations of life sciences and involved in nearly every biological process. Both DNA and protein are the most important part and the most mysterious part of life. Many problems about them remained to be explained. This provided opportunity for me to do research for the progress of life sciences. Certainly, the application in Public Health is also what I concern and I’ve read many works on it during my research work.
Besides academic study and experimental training, I also benefited a lot from the social work I have done during my college life. I led a group of volunteers to provide service to the guests on the occasion of the 100th Anniversary of Peking University. We did a good job and thus were praised by the supervisor. With this experience, my team spirit and leadership, which may contribute a lot to my future research, have been greatly improved.
This summer, I’ll graduate from Peking University and I’d like to further my study in a college with better research doctrine and advanced experimental
出国留学个人陈述personal statement 案例模板21篇(主要生物类PS)
