胰高血糖素样肽-1对原发性高血压大鼠心肌微血管损伤的
保护作用
丁同斌;法宪恩;简立国;刘畅;赵江涛;刘士超
【期刊名称】《中华实验外科杂志》 【年(卷),期】2015(032)012
【摘要】目的 观察胰高血糖素样肽-1(GLP-1)对原发性高血压大鼠心肌微血管损伤的保护作用.方法 120只SHR大鼠随机平均分为4组,分别用于药效实验和细胞实验的对照组和实验组(30只).药效试验:实验组皮下注射艾塞那肽(GLP-1类似物),每日1次,连续7d,对照组注射生理盐水,第8天测定并比较两组大鼠血压、心率、左心室厚度、微动脉密度、壁腔比值、毛细血管密度和内皮细胞面积的差异.细胞实验:提取实验组和对照组大鼠的心肌微血管内皮细胞(CMECs),消化后接种于6孔板,传2～3代,对照组常规细胞培养,研究组采用GLP-1预处理培养,超氧化物试剂盒检测活性氧(ROS)生成,原位缺口末端标记法(TUNEL)法检测CMECs凋亡.结果 药效试验:实验组给药后血压(127.81±10.41) mmHg[1 mmHg=0.133 kPa,P＜0.05]、心率(363.1±15.9)次/分(P＜0.01)以及微动脉特征指标左心室厚度(10.71±1.20)mm(P＜0.05)、微动脉密度4.48±2.21 (P ＜0.05)、壁腔比值1.33 ±0.18(P ＜0.05)和内皮细胞面积17.10 ±5.27(P ＜0.01)较对照组均显著降低,但毛细血管密度显著升高159.7±50.1(P ＜0.05).细胞试验:实验组ROS荧光强度0.25,明显低于对照组(P＜0.05),CMECs凋亡率(39.30 ±3.12)％,明显高于对照组(x2=10.67,P＜0.05).结论 GLP-1干预能够逆转原发性高血压大鼠左心室肥厚,改善其心脏功能,并降低SHR大鼠壁腔比值,增加毛细血管密度和病变细胞凋亡率,对原发性高血压患者心肌微血管损伤具有一定的保
护作用.%Objective Explore protective function of glucagon-like peptide-1 (GLP-1) in essential hypertension patients with microvascular injury.Methods 120 SHR rats were divided into 4 groups,which were study groups and control groups of efficacy trials and cell trials.Efficacy trials: study Group's rats were injected subcutaneously exenatide (GLP-1 analogues) ,control Group's rats were injected with saline, compared the blood pressure, heart rate, left ventricular thickness, micro-arterial density, wall cavity ratio, capillary density, endothelial cell area.Cell trials:extracted microvascular endothelial cells (CMECs) of rat heart muscle from control group and study group, then CMECs were inoculated in 6-well plates after digested, cultured 2-3 generation, the control group received conventional cell culture, the study group was cultured with GLP-1 pretreatment.Detected the reactive oxygen species (ROS) fluorescence intensity and CMECs apoptosis.Results Efficacy trials: study group' s blood pressure (127.81 ± 10.41) mmHg [1 mmHg =0.133 kPa, P ＜ 0.05], heart rate (363.1 ± 15.9)/min (P ＜ 0.01), left ventricular thickness (10.71 ± 1.20) mm(P ＜ 0.05), micro-arterial density 4.48 ± 2.21 (P ＜ 0.05), wall cavity ratio 1.33 ± 0.18 (P ＜ 0.05) and endothelial cell area 17.10 ± 5.27 (P ＜ 0.01) were all lower than control group, but capillary density were higher 159.7 ± 50.1 (P ＜ 0.05).Cell test : study group' s ROS fluorescence intensity 0.25 were significantly lower than control group (P ＜ 0.05).but CMECs apoptosis rate (39.30±3.12)％ were
significantly higher control group (x2 =10.67,P＜0.05).Conclusion GLP-1 can reverse left ventricular hypertrophy,improve heart function and reduce wall cavity ratio,increase capillary density and endothelial cells numbers, which can protect myocardial microvascular injury in essential hypertension patients. 【总页数】3页(3086-3088)
【关键词】胰高血糖素样肽-1;原发性高血压;微血管损伤 【作者】丁同斌;法宪恩;简立国;刘畅;赵江涛;刘士超
【作者单位】450014 郑州大学第二附属医院心内科;450014 郑州大学第二附属医院心外科;450014 郑州大学第二附属医院心内科;450014 郑州大学第二附属医院心内科;450014 郑州大学第二附属医院心内科;450014 郑州大学第二附属医院心内科 【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-experimental-surgery_thesis/0201234756038.html 【相关文献】
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胰高血糖素样肽-1对原发性高血压大鼠心肌微血管损伤的保护作用
