当归补血汤对荷瘤小鼠的影响及对环磷酰胺化疗的增效减毒作用
【摘要】 研究当归补血汤对肿瘤的抑制作用及其与免疫功能的关系，并探讨其对环磷酰胺化疗的增效减毒作用。 方法：以荷EL-4瘤株C57BL/6纯系小鼠作为病理模型，皮下肿瘤接种，7 d时随机分为4组，肿瘤模型组，当归补血汤组［24 g/］，CTX组［ mg/］，DBT+CTX组，另设正常对照组。每天游标卡尺测定肿瘤长短径，并观察荷瘤小鼠生存时间，以检测DBT的抑瘤作用；免疫学方法检测DBT的抑瘤作用与机体免疫功能的关系及其对CTX化疗的增效减毒作用。 结果：接受相应处理15 d后，与肿瘤模型组比较，DBT、CTX和DBT+CTX均可显着减缓小鼠肿瘤生长速度，明显延长荷瘤小鼠生存时间。与肿瘤模型组比较，DBT组和DBT+CTX组各项免疫学指标增强，CTX组各项免疫学指标明显降低。与CTX组比较，DBT+CTX组各项免疫学指标均有大幅度改善。结论：DBT可减缓肿瘤生长速度，延长荷瘤小鼠生存时
间，其抑瘤作用可能与其激活机体免疫功能作用有关，并对CTX化疗有增效减毒作用。 【关键词】 抗肿瘤药; 环磷酰胺; 免疫学; 小鼠
Objective: To study the inhibitory effect of Danggui Buxue Tang (DBT), a compound traditional Chinese herbal medicine for supplementing blood, on tumor growth in tumor-bearing mice after inoculation of EL-4 cells, and its immune mechanism as well as its synergic effect in reducing toxicity of cytoxan (CTX).
Methods: Experiment was carried out in tumor-bearing mice after inoculation of EL-4 cells. The mice were randomly divided into four groups after 7 days of the inoculation: untreated group, DBT-treated group ［24 g/(kg·d)］, CTX-treated group ［ mg/(kg·d)］ and DBT plus CTX-treated group, with another ten normal mice as control. Inhibitory
rate of tumor growth, survival time, immune function and variability of blood cells were measured in the mice during the experiment.
Results: After treatment of relevant interventions for 15 days, the tumor in the DBT-treated group, CTX-treated group and DBT plus CTX-treated group grew slower than the untreated group (). Murine survival time in the DBT-treated group, CTX-treated group and DBT plus CTX-treated group was lengthened as compared with that in the untreated group (). Compared with the untreated group, all kinds of immune indexes in the DBT-treated group and DBT plus
CTX-treated group were significantly improved (), while the immune indexes in the CTX-treated group were decreased (). Compared with the CTX-treated group, all kinds of immune indexes in the DBT plus CTX-treated group were significantly
improved (). Conclusions: DBT can enhance the immune function in tumor-bearing mice and the inhibitory effect of DBT on tumor growth is related to the enhanced immune response. DBT can also increase the therapeutic effects and reduce the side effects of CTX.
Keywords: antineoplastic agents; cyclophosphamide; immunology; mice 肿瘤患者常伴有免疫功能低下，且临床又常以放、化疗作为其常规治疗手段，这两种治疗方式对癌细胞和正常细胞缺乏理想的选择作用，由此导致的骨髓抑制和免疫抑制等严重的毒副作用，可促使机体免疫功能进一步降低，往往成为患者被迫放弃治疗的重要原因。因此，保护骨髓，促进造血功能和免疫功能的恢复乃是肿瘤防治工作中的一个重要方面。当归补血汤为一传统补益气血经典方剂，临床应用广泛。我们及他人的研究已证明当归补血汤可以增强正常小鼠免疫功能。本实验以荷EL-4淋巴瘤C57BL/6
小鼠为动物模型，研究当归补血汤对荷瘤小鼠的影响，探讨DBT抑瘤作用与免疫功能的关系。又采用荷瘤小鼠给予环磷酰胺化疗的方法，造成荷瘤小鼠化疗骨髓抑制模型，观察当归补血汤对接受环磷酰胺化疗的荷瘤小鼠骨髓抑制及免疫功能的影响，为临床应用提供更充分的理论依据。 1 材料与方法 实验材料
动物 C57BL/6小鼠，雌雄各半，10～12周龄，g，购自北京大学医学部动物实验研究部；BALB/c小鼠，雌雄各半，10～12周龄，g，购自北京维通利华实验动物技术有限公司。
细胞株 MX-87、EL-4和L929细胞株液氮保存，均为北京大学医学部免疫药理研究室惠赠。
药物 黄芪和当归中药饮片，均购自北京中医药大学国医堂。按传统方法水煎煮2次(黄芪∶当归=5∶1)，混合后加热浓缩成100%，即相当于含生药量1 g/ml，置于4 ℃的冰箱保存备用；注射用环磷酰胺，上海华
当归补血汤对荷瘤小鼠的影响及对环磷酰胺化疗的增效减毒作用
