近红外光谱用于总胆固醇和甘油三酯的快速同时测定
周丽芳;严夏;潘涛;王大伟;姚立军;肖青青
【期刊名称】《临床医学工程》 【年(卷),期】2014(000)006
【摘要】Objective To study the no-reagent rapid simultaneous determination method for the total cholesterol (TC) and triglyceride (TG) of hyperlipidemia indicators based on near-infrared (NIR) spectroscopy. Methods NIR spectroscopy combined with partial least squares (PLS) regression was used to establish the quantitative analysis model of TC and TG for human serum samples, and the modeling stability was discussed on the basis of varied partitioning of the calibration and prediction sets. Results By comparison and validation, the composite waveband (780~1 878 nm, 2 100~2 320 nm) achieved good prediction accuracy and stability. As for TC, TG indicators, the optimal numbers of PLS factors were 13, 15, and the validation root mean square error of prediction were 0.394, 0.228 (mmol·L-1), and the validation correlation coefficients of prediction were 0.937, 0.982, respectively. The spectral predicted values showed good consistent and correlation with the conventional measured values. The specificity and sensitivity for quantitative identification of hyperlipidemia achieved 91.5%and 92.1%, respectively. Conclusions The results show that NIR spectroscopy can provide a potential simple method suitable for detecting hyperlipidemia
近红外光谱用于总胆固醇和甘油三酯的快速同时测定
